Custom Peptide Synthesis

We provide high-quality peptide synthesis service with a 98% peptide synthesis success rate, which is well above the industry standard of 75%. We are committed to Total Quality Management (TQM) to ensure our customers’ complete satisfaction. TQM includes MS and HPLC analyses performed after the completion of peptide synthesis, purification, and QC (quality control) steps. These analyses are followed by independent QA (quality assurance) procedures, which double guarantee the highest quality possible for every delivered peptide.

Peptide Synthesis Capacity and Capability

Our custom peptides are synthesized with peptide synthesizers, HPLCs, and mass spectrometers capable of producing over 10,000 peptides per month. Other features of our capacity and capability include:

• Capacity: 10,000 peptides / month.
• Flexible quantities: From mg to kg.
• Flexible purities: Crude, desalt, and from 70% to 98% purity.
• Synthesis technologies: Combination of solid phase and liquid phase synthesis, and microwave and ligation technologies
• Modifications: Include phosphorylation, methylation, acetylation, amidation, stable isotope labeling, and many more.
• Quality control: MS and HPLC analyses after each synthesis step, plus additional QA procedures for every peptide.

Range of Services:
Our staff is experienced in the synthesis of high-quality peptides with various modifications. With over 200 units of analytical and preparative HPLC, we can provide peptides from small scale (mgs) to large scale (kgs) in very fast delivery:

• Simple to complex and Linear to cyclized
• Long sequences peptide up to 140 mer
• From small to large scale
• Different purity level (desalted, >75%, >85%, >90%, >95%, >98% and even above 99%)
• Widest variety of modifications (from biotinylation to phosphorylation, to dye labeling, and much more…)
• Peptides – Protein Bioconjugation
• Purification at different scales
• Highest success rate in the industry

Even after multiple rounds of purification, small amounts of impurities may still exist in the final peptide product. Therefore, ORANGE PEPTIDE uses reversed-phase high-performance liquid chromatography (RP-HPLC) to analyze the purity of each custom peptide.

ESI-MS is an analytical tool used to study macromolecules, where the mass-to-charge ratios (m/z) of all ionized analytes are respectively determined. As one of the widely used soft ionization techniques, electrospray ionization produces a whole charged ion from the solution of analytes without fragmentation of the macromolecule, which provides a reliable and accurate way to measure the molecular weight of the macromolecule. Generally, peptides can be ionized into multiple-charged states, either positively or negatively, depending on the number of basic and acidic residues in the sequence.

The ionized peptides are then transferred into a mass analyzer such as a quadrupole or a time-of-flight (TOF), which can separate all charged ions according to their m/z. All these isolated ions are respectively detected by an ion detector such as an electron multiplier and finally are converted into a visual graph called a mass spectrum, which is the plot of ion abundance vs. m/z value of all detected ions. The mass spectrum displays all detected m/z signals and the relative intensity of the ions, which is relative to the highest m/z peak. The highest peak is set as 100%. For most peptides, the mass spectrum displays more than one m/z peak representing a series of charge states. Theoretically the m/z values of these peaks should all result in the same calculated molecular mass, however, calculations from these peaks actually vary with each other slightly due to the limited resolution of the instrument. In our MS report, the calculated molecular weight from the most abundant m/z peak of the analyte is utilized to largely eliminate variations from the instrument. We perform regular cleaning and calibration of mass spectrometers to ensure the mass accuracy and reproducibility of MS analyses.